The development of a mechano-biological assessment of leukemia cells using optical tweezers

Eric Brost; Jamison Brooks; Adam Green; Susanta Hui

doi:10.1117/12.2541338

17 February 2020 The development of a mechano-biological assessment of leukemia cells using optical tweezers

Eric Brost, Jamison Brooks, Adam Green, Susanta Hui

Proceedings Volume 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII; 112431Z (2020) https://doi.org/10.1117/12.2541338
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Patients with B-ALL (Ph +ve) acute lymphoblastic leukemia are at high risk of relapse and mortality. We seek to establish a mechano-biological testing technique to assess biophysical properties of cell motility, potentiating the ability to distinguish between high and low risk leukemia populations and behaviors. Two experiments were performed to test the mechano-biological behavior of B-ALL using the ThorLabs modular optical tweezers and a microfluid chemotaxis chamber. The first experiment measured the relative mechanical energy carried by a cell in the optical trap, which was performed on B-ALL in control conditions and under SDF1 chemotaxis. The relative mechanical energy was found through an extension of the back-focal-plane calibration method for optical tweezers, and assumed that increased cellular activity manifests as random movements. There was no discernable difference in the relative mechanical energies between the control B-ALL, B-ALL under SDF1 chemotaxis, and B-ALL in the presence of mesenchymal stem cells. The second experiment quantified the real-time migrational force of B-ALL under SDF1 chemotaxis. This was found through prior calibration of the optical tweezers through determination of the terms κ and β. Of the viable measurements, 3 of 8 cells exhibited a significant force towards the SDF1 gradient. Further experimentation is necessary to normalize the experimental set-up conditions, and increase the number of viable measurements during cell migration.

Citation Download Citation

Eric Brost, Jamison Brooks, Adam Green, and Susanta Hui "The development of a mechano-biological assessment of leukemia cells using optical tweezers", Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112431Z (17 February 2020); https://doi.org/10.1117/12.2541338

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