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9 March 2020 Spectral phasors by the sin-cos filter method in a two-photon excitation microscope (Conference Presentation)
Enrico Gratton, Alexander Dvornikov
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Proceedings Volume 11244, Multiphoton Microscopy in the Biomedical Sciences XX; 1124404 (2020) https://doi.org/10.1117/12.2546619
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
None of the methods used for hyperspectral imaging work for strongly scattering tissues. In this presentation we show that using the spectral phasor approach we could obtain the spectral phasor representation at each pixel of the image using two-photon excitation and a special filter in the emission that reproduce the sine and cosine transform that is needed to measure the spectral phasor in highly scattering tissues.
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Enrico Gratton and Alexander Dvornikov "Spectral phasors by the sin-cos filter method in a two-photon excitation microscope (Conference Presentation)", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 1124404 (9 March 2020); https://doi.org/10.1117/12.2546619
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KEYWORDS
Microscopes
Optical filters
Prisms
Modulation
Scattering
Tissues
Cameras
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