Multiphoton and confocal imaging of precisely cut oral cancer tumor sections for personalized medicine

Shu-Jen Chiang; Po-Hang Tseng; Hsu-Cheng Huang; Shih-Chi Chen; Shean-Jen Chen; Mei-Chun Lin; Pei-Jen Lou M.D.; Chen-Yuan Dong

doi:10.1117/12.2545617

21 February 2020 Multiphoton and confocal imaging of precisely cut oral cancer tumor sections for personalized medicine

Shu-Jen Chiang, Po-Hang Tseng, Hsu-Cheng Huang, Shih-Chi Chen, Shean-Jen Chen, Mei-Chun Lin, Pei-Jen Lou M.D., Chen-Yuan Dong

Author Affiliations +

Proceedings Volume 11244, Multiphoton Microscopy in the Biomedical Sciences XX; 1124427 (2020) https://doi.org/10.1117/12.2545617
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Traditionally, testing of therapeutic agents uses two-dimensional cell cultures which does not recapitulate the complex, three-dimensional architecture of tissues. In the case of cancer drug development, immunotherapy (IT) emerged as an effective treatment strategy for the patients who respond to therapy. However, the overall patient response rate for immunotherapy is around 25%. Therefore, there is a need to develop more effective drug testing platform for cancer patients. In this work, we develop three-dimensional tissue cultures of human oral cancer slices. We observed the growth dynamics of the tissue sections for up to 28 days. Their viability over extended periods suggest that the tissue sections may be used for personalized drug testing.

Citation Download Citation

Shu-Jen Chiang, Po-Hang Tseng, Hsu-Cheng Huang, Shih-Chi Chen, Shean-Jen Chen, Mei-Chun Lin, Pei-Jen Lou M.D., and Chen-Yuan Dong "Multiphoton and confocal imaging of precisely cut oral cancer tumor sections for personalized medicine", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 1124427 (21 February 2020); https://doi.org/10.1117/12.2545617

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