A multiplexed confocal FLIM microscope with 4-taps time-gated imager

Morgan Richards; Yuya Shirakawa; Fares Badr; Keiichiro Kagawa; Shoji Kawahito; Qiyin Fang

doi:10.1117/12.2550208

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17 February 2020 A multiplexed confocal FLIM microscope with 4-taps time-gated imager

Morgan Richards, Yuya Shirakawa, Fares Badr, Keiichiro Kagawa, Shoji Kawahito, Qiyin Fang

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Proceedings Volume 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII; 1124512 (2020) https://doi.org/10.1117/12.2550208
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Combined with confocal imaging, Fluorescence lifetime imaging microscopy (FLIM) can achieve 3-dimensional optical sectional capability with sub-nanosecond lifetime information. As confocal FLIM acquires multi-dimensional data 4D (3D space + time), it is inherently slow. Recent developments in lock-in pixel imagers with time gated pixels show such detectors are capable of collecting as many as 8-time gates in a single pixel cycle. We present a multiplexed confocal FLIM microscope, equipped with a 4-taps time-gated lock-in pixel imager. The multiplexing setup allows the use of the sparse array with sub-nanosecond time-gating to achieve high throughput FLIM acquisition.

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Morgan Richards, Yuya Shirakawa, Fares Badr, Keiichiro Kagawa, Shoji Kawahito, and Qiyin Fang "A multiplexed confocal FLIM microscope with 4-taps time-gated imager", Proc. SPIE 11245, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII, 1124512 (17 February 2020); https://doi.org/10.1117/12.2550208

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