Planar plasmonic antenna arrays resolve transient nanoscopic heterogeneities in biological membranes

Pamina M. Winkler; Felix Campelo; Marina I. Giannotti; María F. García-Parajo

doi:10.1117/12.2543726

13 February 2020 Planar plasmonic antenna arrays resolve transient nanoscopic heterogeneities in biological membranes

Pamina M. Winkler, Felix Campelo, Marina I. Giannotti, María F. García-Parajo

Proceedings Volume 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII; 112460F (2020) https://doi.org/10.1117/12.2543726
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We introduce an innovative design of planar plasmonic nanogap antenna arrays and demonstrate its potential to study the spatiotemporal organization of mimetic biological membranes at the nanoscale. We exploit our novel nanogap antenna platform with different nanogap sizes (10-45 nm) combined with fluorescence correlation spectroscopy to reveal the existence of nanoscopic domains in mimetic biological membranes. Our approach takes advantage of the highly enhanced and confined excitation light provided by the antennas together with their extreme planarity to investigate membrane regions as small as 10 nm in size with microsecond temporal resolution. We first demonstrate the ultra-high confinement of photonic antennas on biological membranes. Moreover, we show that cholesterol slows down the diffusion of individual fluorescent molecules embedded in the lipid bilayer, consistent with the formation of nanoscopic domains enriched by cholesterol. Incorporation of hyaluronic acid (HA) to the ternary lipid mixture further slows down molecular diffusion, suggesting a synergistic effect of cholesterol and HA on the dynamic partitioning of mimetic biological membranes.

Conference Presentation

Citation Download Citation

Pamina M. Winkler, Felix Campelo, Marina I. Giannotti, and María F. García-Parajo "Planar plasmonic antenna arrays resolve transient nanoscopic heterogeneities in biological membranes", Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 112460F (13 February 2020); https://doi.org/10.1117/12.2543726

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