CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 11246 > Article
Presentation
9 March 2020 3D single molecule localization microscopy based on time modulated illumination (Conference Presentation)
Pierre Jouchet, Clément Cabriel, Nicolas Bourg, Marion Bardou, Christian Poüs, Emmanuel Fort, Sandrine Leveque-Fort
Author Affiliations +
Proceedings Volume 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII; 112460Q (2020) https://doi.org/10.1117/12.2545196
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
We propose Modloc, a new single molecule localization strategy based on a time signature. By shifting a structured excitation pattern, it induces a time modulated emission of the fluorophores where the phase holds its position. An efficient demodulation optical assembly compatible with short emitters On-time has been designed. Performances of ModLoc, will be discussed, in particular its 2 fold lateral precision improvement and its robustness to optical aberrations. By applying the modulation in a tilted direction, we will show that an almost isotropic 3D localization (~11 nm) is obtained over the whole capture range up to 30 µm in depth
Conference Presentation
© (2020) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Pierre Jouchet, Clément Cabriel, Nicolas Bourg, Marion Bardou, Christian Poüs, Emmanuel Fort, and Sandrine Leveque-Fort "3D single molecule localization microscopy based on time modulated illumination (Conference Presentation)", Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 112460Q (9 March 2020); https://doi.org/10.1117/12.2545196
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Modulation
Molecules
Microscopy
Point spread functions
Cameras
Demodulation
Fringe analysis
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top