Quantitative phase imaging of neuronal movement during action potential (Conference Presentation)

Kevin C. Boyle; Tong Ling; Valentina Zuckerman; Thomas Flores; Daniel V. Palanker

doi:10.1117/12.2545250

11 March 2020 Quantitative phase imaging of neuronal movement during action potential (Conference Presentation)

Kevin C. Boyle, Tong Ling, Valentina Zuckerman, Thomas Flores, Daniel V. Palanker

Proceedings Volume 11249, Quantitative Phase Imaging VI; 112490S (2020) https://doi.org/10.1117/12.2545250
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Nanometer-scale deformations of the neuron accompany the action potential. These displacements are measured using a fast quantitative phase microscope and averaged in synchrony with optogenetic stimulation of cultured neurons. The phase movie is further processed by leveraging the spatial and temporal distribution of the spiking signal to detect and segment the separate action potentials in individual cells. An accompanying confocal fluorescence microscopy provides the 3-D cell shape for calibration of the refractive index to calculate the mechanical displacements from the optical phase. Together, these results illuminate the underlying mechanism of the cellular deformations and techniques for achieving all-optical single spike detection.

Conference Presentation

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Kevin C. Boyle, Tong Ling, Valentina Zuckerman, Thomas Flores, and Daniel V. Palanker "Quantitative phase imaging of neuronal movement during action potential (Conference Presentation)", Proc. SPIE 11249, Quantitative Phase Imaging VI, 112490S (11 March 2020); https://doi.org/10.1117/12.2545250

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KEYWORDS

Action potentials

Phase imaging

Neurons

Brain mapping

Cameras

Data analysis

Image processing

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