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11 March 2020 Quantifying myelination of single neurons using Spatial Light Interference Microscopy (SLIM) (Conference Presentation)
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Proceedings Volume 11249, Quantitative Phase Imaging VI; 112490T (2020)
Event: SPIE BiOS, 2020, San Francisco, California, United States
Although both neurons and oligodendrocytes have been well studied individually, very little is known about how they interact with each other. New methods are needed to further study the intricacies of this interplay in terms of cellular and molecular dynamics. Spatial Light Interference Microscopy (SLIM) is a quantitative phase imaging technique that generates phase maps related to the dry mass content of the sample. In this work, we study the ability of SLIM to quantify myelination at the axonal level. We imaged a series of cocultures comprising hippocampal neurons and oligodendrocytes, of varying densities, using SLIM, and evaluated dry mass formation and growth of myelin.
Conference Presentation
© (2020) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Michael J. Fanous, Young Jae Lee, Catherine A. Best-Popescu, Allison Louie, Andrew Steelman, Megan P. Caputo, Laurie A. Rund, Rodney W. Johnson, Tapas Das, Matthew J. Kuchan, and Gabriel Popescu "Quantifying myelination of single neurons using Spatial Light Interference Microscopy (SLIM) (Conference Presentation)", Proc. SPIE 11249, Quantitative Phase Imaging VI, 112490T (11 March 2020);

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