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14 February 2020 Computational microscopy for quantitative phase imaging and refractive index tomography using annular illumination
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Proceedings Volume 11249, Quantitative Phase Imaging VI; 112491F (2020) https://doi.org/10.1117/12.2543597
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
In this work, we demonstrate a computational microscopy for quantitative phase imaging and refractive index tomography using annular illumination. By employing a physical annular plate or a programmable annular LED unit, the quantitative phase images of a thin phase object or large-volume three-dimensional (3D) refractive index (RI) distributions of thick object can be rapidly characterize and recovered. This annular illumination scheme optimally encodes both low- and high-spatial frequency for quantitative phase and RI information across the entire 3D volume using a small number of intensity measurements. We also give both quantitative phase and 3D RI experiment results based on various biological samples, and this computational microscopy approach shows promise as a powerful high-speed, label-free tool for biomedical applications and possibility of widespread adoption of phase imaging in the morphology study of cellular processes and biomedical community.
© (2020) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jiaji Li, Alex Matlock, Yunzhe Li, Qian Chen, Lei Tian, and Chao Zuo "Computational microscopy for quantitative phase imaging and refractive index tomography using annular illumination", Proc. SPIE 11249, Quantitative Phase Imaging VI, 112491F (14 February 2020); https://doi.org/10.1117/12.2543597
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