Characterization and compensation of wavelength dependent aberrations in hyperspectral quantitative phase imaging with digital holographic microscopy

Nikolai Storm; Álvaro Barroso; Jürgen Schnekenbuerger; Björn Kemper

doi:10.1117/12.2545981

14 February 2020 Characterization and compensation of wavelength dependent aberrations in hyperspectral quantitative phase imaging with digital holographic microscopy

Nikolai Storm, Álvaro Barroso, Jürgen Schnekenbuerger, Björn Kemper

Author Affiliations +

Proceedings Volume 11249, Quantitative Phase Imaging VI; 112491P (2020) https://doi.org/10.1117/12.2545981
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Multispectral quantitative phase imaging (QPI) with digital holographic microscopy (DHM) has been demonstrated to be a versatile tool to access wavelength dependent optical properties of technical and biological specimens as well as to reduce coherence induced image disturbances. However, when hyperspectral DHM is combined with microscopes and tunable lasers with a large spectral range, chromatic aberrations of the optical imaging system have to be considered. We have analyzed wavelength dependent defocusing effects and changes of the optical magnification in the related refocused holographic images in lens-based multi-wavelength DHM and show that both effects can be efficiently numerically compensated.

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Nikolai Storm, Álvaro Barroso, Jürgen Schnekenbuerger, and Björn Kemper "Characterization and compensation of wavelength dependent aberrations in hyperspectral quantitative phase imaging with digital holographic microscopy", Proc. SPIE 11249, Quantitative Phase Imaging VI, 112491P (14 February 2020); https://doi.org/10.1117/12.2545981

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