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10 March 2020 FluorATOM: high-throughput imaging flow cytometry for synchronized biophysical and biomolecular phenotyping (Conference Presentation)
Kelvin Lee, Maolin Wang, Isabella Cheuk, Vivian Shine, Ava Kwong, Kenneth Wong, Hayden So, Kevin Tsia
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Proceedings Volume 11250, High-Speed Biomedical Imaging and Spectroscopy V; 112500L (2020) https://doi.org/10.1117/12.2548252
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
Multiplexed asymmetric-detection time-stretch optical microscopy (multi-ATOM) has recently been developed to enable high-throughput quantitative phase imaging flow cytometry, from which single-cell biophysical properties can be measured at large scale. However, it lacks the ability to link such biophysical knowledge to biomolecular signatures at the single-cell precision for validation and correlative multi-scale single-cell analysis. We report a high-throughput multimodal system that integrates multi-ATOM with multiplexed 1-D fluorescence imaging/detection, termed FluorATOM; and applied it to perform synchronized biophysical and biomolecular phenotyping of rare breast circulating tumor cells detected in peripheral blood in a mouse xenograft at a throughput of >10,000 cell/sec.
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Kelvin Lee, Maolin Wang, Isabella Cheuk, Vivian Shine, Ava Kwong, Kenneth Wong, Hayden So, and Kevin Tsia "FluorATOM: high-throughput imaging flow cytometry for synchronized biophysical and biomolecular phenotyping (Conference Presentation)", Proc. SPIE 11250, High-Speed Biomedical Imaging and Spectroscopy V, 112500L (10 March 2020); https://doi.org/10.1117/12.2548252
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KEYWORDS
Flow cytometry
Blood
Multiplexing
Ultrafast imaging
Biological research
Fluorescence spectroscopy
In vitro testing
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