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10 March 2020 Full-field interferometric imaging of neuron deformation during action potential (Conference Presentation)
Tong Ling, Kevin C. Boyle, Valentina Zuckerman, Thomas Flores, Daniel V. Palanker
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Proceedings Volume 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020; 112511Q (2020) https://doi.org/10.1117/12.2543625
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
We report the full-field imaging of the mechanical deformations accompanying the action potential in primary cortical neurons using ultrafast quantitative phase imaging (QPI) with a temporal resolution of 0.1 ms and a membrane displacement sensitivity of <0.2 nm per pixel. The average displacements were ~0.7 nm on cell somas and ~0.5 nm on neurites. Finite element modeling based on the 3D shape extracted from confocal imaging and on scaling of the surface tension with trans-membrane voltage yielded the deformation map during action potential, which matched the features of the experimental results, including the displacement amplitude, time course, and spatial distribution.
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Tong Ling, Kevin C. Boyle, Valentina Zuckerman, Thomas Flores, and Daniel V. Palanker "Full-field interferometric imaging of neuron deformation during action potential (Conference Presentation)", Proc. SPIE 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020, 112511Q (10 March 2020); https://doi.org/10.1117/12.2543625
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KEYWORDS
Action potentials
Neurons
Interferometry
3D modeling
Finite element methods
Axons
Imaging arrays
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