Translator Disclaimer
10 March 2020 Spatial frequency projection imaging of coherent anti-Stokes Raman scattering (SPIFI-CARS) (Conference Presentation)
Author Affiliations +
Conventional CARS microscopy requires scanning a point focus through the specimen limits imaging speed. We present a spatial frequency projection imaging (SPIFI) method for CARS microscopy to spatially multiplex CARS microscopy. A spinning disk modulator is used to rapidly modulate the Stokes field with a rapidly swept spatially periodic transmission grating. SPIFI-CARS images are obtained by Fourier transforming the single pixel signal. Images of CARS and second harmonic generation from histological slices will be presented. The physics of image formation and the impact of multiplexing on SNR will be discussed. Prospects for scaling to high speed CARS imaging will be discussed.
Conference Presentation
© (2020) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Sandro Heuke, Siddharth Sivankutty, Camille Scotte, Patrick A. Stockton, Randy A. Bartels, Anne Sentenac, and Hervé Rigneault "Spatial frequency projection imaging of coherent anti-Stokes Raman scattering (SPIFI-CARS) (Conference Presentation)", Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 1125203 (10 March 2020);

Back to Top