Tracking bacteria clearance using gold nanoparticles for wound therapy applications

Menachem Motiei; Oshra Betzer; Tamar Sadan; Neta Zilony; Guy Topaz; Rachela Popovtzer; Moris Topaz; Dror Fixler

doi:10.1117/12.2548441

21 February 2020 Tracking bacteria clearance using gold nanoparticles for wound therapy applications

Menachem Motiei, Oshra Betzer, Tamar Sadan, Neta Zilony, Guy Topaz, Rachela Popovtzer, Moris Topaz, Dror Fixler

Proceedings Volume 11254, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII; 112541H (2020) https://doi.org/10.1117/12.2548441
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Bacterial infection and biofilm buildup is a major challenge for wound healing. In the present work we developed a method to track bacteria in vivo in order to analyze the progress of the healing. Regulated irrigation combined with negative pressure (RI-NPT) is a nonpharmaceutical therapeutic strategy for reducing bacterial load in acute and chronically infected wounds. Here, we analyzed RI-NPT hydrokinetics and efficacy of bacterial load reduction in wounds. Escherichia coli were loaded with gold nanoparticles, in order to make them "visible" and trackable using computed tomography (CT). Bacteria were tracked using CT in a low-flow rat wound model over time. CT tracking revealed that while regulated negative pressure-assisted wound therapy reduced bacterial load to a limited extent (5%), RI-NPT significantly increased bacterial outflow and clearance (by 45%). This nanotechnology-based approach demonstrates the ability to track bacteria in vivo at real time and also the ability of the RI-NPT technology to effectively reduce bacterial load.

Citation Download Citation

Menachem Motiei, Oshra Betzer, Tamar Sadan, Neta Zilony, Guy Topaz, Rachela Popovtzer, Moris Topaz, and Dror Fixler "Tracking bacteria clearance using gold nanoparticles for wound therapy applications", Proc. SPIE 11254, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII, 112541H (21 February 2020); https://doi.org/10.1117/12.2548441

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available