In-vitro detection of polypeptide conformations at attomolar concentration using an ultrafast laser and surface-enhanced infrared absorption spectroscopy on a single resonant nanoantenna (Conference Presentation)

Florian Mörz; Rostyslav Semenyshyn; Tobias R. J. Steinle; Monica Ubl; Mario Hentschel; Frank F. Neubrech; Harald Giessen

doi:10.1117/12.2545928

10 March 2020 In-vitro detection of polypeptide conformations at attomolar concentration using an ultrafast laser and surface-enhanced infrared absorption spectroscopy on a single resonant nanoantenna (Conference Presentation)

Florian Mörz, Rostyslav Semenyshyn, Tobias R. J. Steinle, Monica Ubl, Mario Hentschel, Frank F. Neubrech, Harald Giessen

Author Affiliations +

Proceedings Volume 11257, Plasmonics in Biology and Medicine XVII; 112570J (2020) https://doi.org/10.1117/12.2545928
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Infrared spectroscopy enables the investigation of conformational protein changes, associated with human diseases, such as Diabetes II or Alzheimer’s disease. However, the in-vitro investigation of individual molecules remains challenging, but could provide insight into mechanisms leading to structural changes. This is due to the lack of suitable light sources, among other things. Here, we detect polypeptide conformations at attomolar concentration within minutes, exploiting Fourier-transform infrared (FTIR) spectroscopy and the plasmonic enhancement of a single resonant nanoantenna, being enabled by using a highly brilliant, broadband mid-IR laser. We successfully determine polypeptide conformations and compare our results to Globar and synchrotron measurements.

Conference Presentation

Citation Download Citation

Florian Mörz, Rostyslav Semenyshyn, Tobias R. J. Steinle, Monica Ubl, Mario Hentschel, Frank F. Neubrech, and Harald Giessen "In-vitro detection of polypeptide conformations at attomolar concentration using an ultrafast laser and surface-enhanced infrared absorption spectroscopy on a single resonant nanoantenna (Conference Presentation)", Proc. SPIE 11257, Plasmonics in Biology and Medicine XVII, 112570J (10 March 2020); https://doi.org/10.1117/12.2545928

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