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10 March 2020 High-throughput and label-free imaging of individual biological nanoparticles at high-resolution (Conference Presentation)
Celalettin Yurdakul, Oguzhan Avci, Alex C. Matlock, Lei Tian, Selim Ünlü
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Proceedings Volume 11258, Frontiers in Biological Detection: From Nanosensors to Systems XII; 112580H (2020) https://doi.org/10.1117/12.2545232
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
Morphological parameters of biological nanoparticles (BNPs) have strong implications on their fate and functionality in vivo e.g., circulation, biodistribution, and clearance. Although interferometric scattering microscopy modalities have demonstrated the label-free detection of sub-100 nm BNPs including viruses and exosomes; they have an insufficient spatial resolution roughly limited to the illumination wavelength. Here, we introduce computational imaging to interferometric scattering microscopy with asymmetric illumination and demonstrate a two-fold resolution enhancement. We demonstrate high-resolution imaging of nanoparticles across a large field-of-view of 100 µm × 100 µm. This novel imaging platform enables ultrasensitive and label-free morphological visualization of low-index sub-diffraction-limited BNPs in a high-throughput manner at subwavelength resolution.
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Celalettin Yurdakul, Oguzhan Avci, Alex C. Matlock, Lei Tian, and Selim Ünlü "High-throughput and label-free imaging of individual biological nanoparticles at high-resolution (Conference Presentation)", Proc. SPIE 11258, Frontiers in Biological Detection: From Nanosensors to Systems XII, 112580H (10 March 2020); https://doi.org/10.1117/12.2545232
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KEYWORDS
Nanoparticles
Interferometry
Microscopy
Scattering
Image resolution
Spatial resolution
In vivo imaging
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