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21 February 2020 Use of peptide capture agents in porous silicon biosensors
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Abstract
We report the use of a peptide-based capture agent as an alternative to antibodies and nucleic acid-based bioreceptors for porous silicon biosensors. Click chemistry is employed to attach azide-functionalized streptavidin-binding peptides to alkyne-modified porous silicon films. The attachment of the streptavidin-binding peptide and subsequent detection of streptavidin molecules are verified using optical reflectance and Fourier transform infrared spectroscopy measurements.
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Tengfei Cao, Rabeb Layouni, Matthew B. Coppock, Paul E. Laibinis, and Sharon M. Weiss "Use of peptide capture agents in porous silicon biosensors", Proc. SPIE 11258, Frontiers in Biological Detection: From Nanosensors to Systems XII, 112580L (21 February 2020); https://doi.org/10.1117/12.2552932
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