Digital micromirror device based angle-multiplexed optical diffraction tomography for high throughput 3D imaging of cells

Yanping He; Yijin Wang; Renjie Zhou

doi:10.1117/12.2547017

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28 February 2020 Digital micromirror device based angle-multiplexed optical diffraction tomography for high throughput 3D imaging of cells

Yanping He, Yijin Wang, Renjie Zhou

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Proceedings Volume 11294, Emerging Digital Micromirror Device Based Systems and Applications XII; 1129402 (2020) https://doi.org/10.1117/12.2547017
Event: SPIE OPTO, 2020, San Francisco, California, United States

Abstract

The development of high throughput three-dimensional (3D) microscopic imaging technique is important for studying cell physiology and early-stage disease diagnoses. Here we propose and demonstrate a digital micromirror device (DMD) based angle-multiplexed high-speed optical diffraction tomography (ODT) technique. Using this ODT technique, we have achieved 3D imaging of cells at over 600 tomogram/second speed, which is 10-100 times faster than current ODTbased 3D cell imaging techniques. We envision that this high-speed ODT system will enable many cutting-edge biomedical applications, such as capturing millisecond scale cell dynamics in 3D space and high throughput 3D imaging of large cell populations.

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Yanping He, Yijin Wang, and Renjie Zhou "Digital micromirror device based angle-multiplexed optical diffraction tomography for high throughput 3D imaging of cells", Proc. SPIE 11294, Emerging Digital Micromirror Device Based Systems and Applications XII, 1129402 (28 February 2020); https://doi.org/10.1117/12.2547017

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