Micro-computed tomography imaging of a rodent model of Chronic Obstructive Pulmonary Disease (COPD)

Nancy L. Ford; Ian Lee; Anthony Tam; Don D. Sin

doi:10.1117/12.2549805

28 February 2020 Micro-computed tomography imaging of a rodent model of Chronic Obstructive Pulmonary Disease (COPD)

Nancy L. Ford, Ian Lee, Anthony Tam, Don D. Sin

Author Affiliations +

Proceedings Volume 11317, Medical Imaging 2020: Biomedical Applications in Molecular, Structural, and Functional Imaging; 113172F (2020) https://doi.org/10.1117/12.2549805
Event: SPIE Medical Imaging, 2020, Houston, Texas, United States

Abstract

Chronic obstructive pulmonary disease is projected to become the 3rd leading cause of death worldwide by 2030. Currently, 200 million people worldwide have been diagnosed with COPD, and many more are living with undiagnosed disease. COPD has no cure and no drugs that lead to improvements in long-term survival. Drug discovery is challenging due to a poor understanding of COPD pathogenesis. To study COPD, rodent models have been developed, with daily exposures to tobacco cigarette smoke over a 6-month period inducing symptoms. Measurements are typically done on histological slides, assessing airway wall thickening and markers of emphysema. These post-mortem techniques are unable to assess how the disease is progressing or how these observed structural changes impact lung function. To identify changes in lung structure and function in a smoking exposure model, we used respiratory-gated micro-computed tomography (micro-CT) and image-based measurements of lung structure and function. Micro-CT imaging was performed in anesthetized, free-breathing mice at baseline. The mice were then subjected to 6-months of exposure to tobacco cigarettes or ambient air, and rescanned. We also performed post-mortem lung compliance tests on 3-month smoke-exposed and age-matched control mice. Significant differences between smoke-exposed and control mice were observed for lung volume and functional residual capacity, which correlate well with the results of the lung compliance testing. In vivo respiratory-gated micro-CT in free-breathing animals is sensitive to changes in lung structure and function resulting from exposure to tobacco cigarette smoke, and is an effective tool to monitor the development of COPD in rodent models.

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Nancy L. Ford, Ian Lee, Anthony Tam, and Don D. Sin "Micro-computed tomography imaging of a rodent model of Chronic Obstructive Pulmonary Disease (COPD)", Proc. SPIE 11317, Medical Imaging 2020: Biomedical Applications in Molecular, Structural, and Functional Imaging, 113172F (28 February 2020); https://doi.org/10.1117/12.2549805

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