Effects of radiation exposure on dermal collagen: A multi modal approach

Anna Maeva; Jeffrey Bamber; Inna Seviaryna; Colin Hopper; Conal Perrett; Laurent Bozec

doi:10.1117/12.2549843

16 March 2020 Effects of radiation exposure on dermal collagen: A multi modal approach

Anna Maeva, Jeffrey Bamber, Inna Seviaryna, Colin Hopper, Conal Perrett, Laurent Bozec

Author Affiliations +

Proceedings Volume 11319, Medical Imaging 2020: Ultrasonic Imaging and Tomography; 113190P (2020) https://doi.org/10.1117/12.2549843
Event: SPIE Medical Imaging, 2020, Houston, Texas, United States

Abstract

Intense pulsed light (IPL) is a high-intensity treatment for skin disorders and ageing. As this treatment regime is often poorly regulated and inadequately studied, we investigate IPL as a cosmetic device and its effects on dermal collagen components of the skin. Biopsies from the back-neck folds of a 4-week-old, 25 kg large white pig were irradiated with intense pulsed light (IPL) (l= 584 nm) at an increased radiation dose of 40 J/cm² once, thrice and ten times. Samples were cryo-sectioned (10 μm) and stained with picro sirrus red. Ex-vivo biopsies were assessed with polarized light microscopy (PLM), atomic force microscopy (AFM) and scanning acoustic microscopy. Customized software was used to map the sound speed and attenuation on the ultrasonic images Differences in collagen structure were observed between all three levels of irradiation progressing depth-wise into the epidermis. Ex-vivo porcine tissue demonstrated loss of D-banding and gelatinization with increasing dermal depth with higher intensities. Acoustic microscopy demonstrated a significant decrease in sound speed and attenuation that relates to the number of exposures. Sound speed decreases at much faster rates than attenuation.

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Anna Maeva, Jeffrey Bamber, Inna Seviaryna, Colin Hopper, Conal Perrett, and Laurent Bozec "Effects of radiation exposure on dermal collagen: A multi modal approach", Proc. SPIE 11319, Medical Imaging 2020: Ultrasonic Imaging and Tomography, 113190P (16 March 2020); https://doi.org/10.1117/12.2549843

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