The study of paraffin-embedded tissue using multiphoton microscopy

Anastasia I. Knyazkova; Lyudmila V. Spirina; Viсtor V. Nikolaev; Alexey V. Borisov; Аlexey A. Markelov; Maxim B. Mirochnichenko; Elena S. Sim; Yury V. Kistenev; Polina A. Timoshina; Daria K. Tuchina

doi:10.1117/12.2550754

11 December 2019 The study of paraffin-embedded tissue using multiphoton microscopy

Anastasia I. Knyazkova, Lyudmila V. Spirina, Viсtor V. Nikolaev, Alexey V. Borisov, Аlexey A. Markelov, Maxim B. Mirochnichenko, Elena S. Sim, Yury V. Kistenev, Polina A. Timoshina, Daria K. Tuchina

Author Affiliations +

Proceedings Volume 11322, XIV International Conference on Pulsed Lasers and Laser Applications; 113222J (2019) https://doi.org/10.1117/12.2550754
Event: XIV International Conference on Pulsed Lasers and Laser Applications (AMPL-2019), 2019, Tomsk, Russian Federation

Abstract

The of multiphoton microscopy (MPM) based on two-photon fluorescence and second-harmonic generation is promising for diagnosing neoplastic transformation of tissues. The results of MPM studies of tissues with prostate adenocarcinoma embedded in paraffin blocks are presented. The tissue fluorescence was also analyzed using the fluorescence lifetime (Fluorescence-lifetime imaging microscopy - FLIM) option. The relationships between the fluorescence peculiarities and the second harmonic generation of the prostate gland tissues were obtained for different morphological images.

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Anastasia I. Knyazkova, Lyudmila V. Spirina, Viсtor V. Nikolaev, Alexey V. Borisov, Аlexey A. Markelov, Maxim B. Mirochnichenko, Elena S. Sim, Yury V. Kistenev, Polina A. Timoshina, and Daria K. Tuchina "The study of paraffin-embedded tissue using multiphoton microscopy", Proc. SPIE 11322, XIV International Conference on Pulsed Lasers and Laser Applications, 113222J (11 December 2019); https://doi.org/10.1117/12.2550754

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