Coherent anti-Stokes Raman scattering microscopy through a multimode fiber endoscope

Johanna Trägårdh; Tomáš Pikálek; Mojmír Šerý; Denis Akimov; Tobias Meyer; Jürgen Popp; Tomáš Cižmár

doi:10.1117/12.2555080

1 April 2020 Coherent anti-Stokes Raman scattering microscopy through a multimode fiber endoscope

Johanna Trägårdh, Tomáš Pikálek, Mojmír Šerý, Denis Akimov, Tobias Meyer, Jürgen Popp, Tomáš Cižmár

Proceedings Volume 11359, Biomedical Spectroscopy, Microscopy, and Imaging; 1135907 (2020) https://doi.org/10.1117/12.2555080
Event: SPIE Photonics Europe, 2020, Online Only

Abstract

Advanced wavefront-shaping methods can be used to transform a simple multimode fiber into an ultra-thin laser scanning microscope. Here, we extend this technique to label-free non-linear microscopy with chemical contrast using coherent anti-Stokes Raman scattering (CARS) through a multimode fiber endoscope, which opens up new avenues for instant and in-situ diagnosis of potentially malignant tissue. We use a commercial, 125 μm diameter, 0.29 NA, GRIN fiber as the endoscopic probe. Wavefront shaping on a spatial light modulator is used to create a focus, where the 1-2 ps long pump and Stokes pulses are overlapped in time, which is scanned behind the fiber facet across the sample. The chemical selectivity is demonstrated by imaging 2 μm polystyrene and 2.5 μm PMMA beads with per pixel integration time as low as 1 ms for epi-detection. Epi-detection through the fiber is possible despite the fact that the CARS signal is emitted mainly in the forward direction, away from the fiber facet. Detecting the back-scattered signal from the underlying tissue, requires a large detector aperture to be efficient. By detecting through both the core and the cladding of the fiber, we obtain sufficient detection efficiency.

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Johanna Trägårdh, Tomáš Pikálek, Mojmír Šerý, Denis Akimov, Tobias Meyer, Jürgen Popp, and Tomáš Cižmár "Coherent anti-Stokes Raman scattering microscopy through a multimode fiber endoscope", Proc. SPIE 11359, Biomedical Spectroscopy, Microscopy, and Imaging, 1135907 (1 April 2020); https://doi.org/10.1117/12.2555080

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