Evaluation of germination ability of seeds at different temperatures by biospeckle optical coherence tomography

Yiheng Lim; Hirofumi Kadono

doi:10.1117/12.2555008

2 April 2020 Evaluation of germination ability of seeds at different temperatures by biospeckle optical coherence tomography

Yiheng Lim, Hirofumi Kadono

Author Affiliations +

Proceedings Volume 11363, Tissue Optics and Photonics; 113630B (2020) https://doi.org/10.1117/12.2555008
Event: SPIE Photonics Europe, 2020, Online Only

Abstract

Gemination tests and vigor tests are destructive sampling tests used to ensure the quality of seeds. However, the yields may depend on the conditions of the fields even the quality of seeds were tested. A non-destructive method to inspect the properties of seeds in the fields can improve the yields of crops. A biospeckle OCT was developed to measure the biological activities of germinating seeds. Twenty-nine radish seeds were measured by the OCT under 3 conditions. Nine seeds were treated with hot water to terminate the biological activities before the measurement of OCT and imbibed in room temperatures. Ten seeds were germinated for each condition at room and low temperatures. Low temperatures were to slow down the biological activities. The speckle contrast, defined as the ratio of the standard deviation to the mean of OCT intensities, was used to quantify the biological activities. The modal values of speckle contrast largely increased in 1.8 days when the seeds were germinated at room temperatures. When the seeds were germinated at low temperatures the modal values of speckle contrast took 14 days to increase to the same level of that of the seeds germinated at room temperatures. The modal values of speckle contrast remained low for 14 days of OCT measurements when the seeds were heated. As a conclusion, the results showed that the speckle contrast of OCT intensities quantified the biological activities of seeds during the process of germination and it may be used to evaluate the ability of seed germination.

Conference Presentation

Citation Download Citation

Yiheng Lim and Hirofumi Kadono "Evaluation of germination ability of seeds at different temperatures by biospeckle optical coherence tomography", Proc. SPIE 11363, Tissue Optics and Photonics, 113630B (2 April 2020); https://doi.org/10.1117/12.2555008

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