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The results of an experimental study of changes in the microcirculation of subcutaneous blood flow in a malignant tumor using laser speckle contrast imaging (LSCI) using an optical clearing agent of 70% glycerol solution are presented. In this study, for hemodynamic monitoring we used the speckle contrast method, which is one of the laser speckle imaging methods. This method is based on calculation of contrast of time-averaged dynamic speckles in dependence on the exposure time at the registration of the speckle-modulated images. Analysis of change of hemodynamic was carried out depending on the stage of development of pathologyIn research, we used 15 white laboratory rats Wistar weighing 300-500 g. A tumor was inoculated in laboratory rats by subcutaneous injection of 0.5 ml of a 25% tumor suspension into the area of the shoulder blades in a Hanks solution strain of PC1 alveolar liver cancer. Laboratory animals were divided into three groups, the first included healthy animals, the second - animals with an inoculated tumor 14 days after injection and the third - animals with an inoculated tumor 28 days after injection. It was found that the blood flow rate in pathological group No. 2 was reduced by 25% relative to control measurements of group No. 1, in pathological group No. 3 increased by 20% relative to control measurements. Also, the results showed that the use of a 70% glycerol solution reduced the blood flow velocity in the control group by about 2 times. Moreover, in pathological groups, the effect of the solution on the blood flow is more pronounced, in the group, the measurements on which were carried out 14 days after the inoculation of the tumor, the blood flow rate decreased by 2.8 times, after 28 days - by 4 times. The results obtained in the study of disorders of the microcirculation of the subcutaneous blood flow under the conditions of the development of a malignant tumor show that the development of diseases in animals causes changes in the microcirculatory system, and the use of optical clearing agents demonstrates changes in vascular permeability under conditions of the development of pathologies.
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