Light-field Fourier Microscope (FiMic) for transparent objects (Conference Presentation)

Gabriele Scrofani; Jorge Sola-Pikabea; Emilio Sánchez-Ortiga; Manuel Martínez-Corral; Genaro Saavedra

doi:10.1117/12.2558113

22 April 2020 Light-field Fourier Microscope (FiMic) for transparent objects (Conference Presentation)

Gabriele Scrofani, Jorge Sola-Pikabea, Emilio Sánchez-Ortiga, Manuel Martínez-Corral, Genaro Saavedra

Proceedings Volume 11402, Three-Dimensional Imaging, Visualization, and Display 2020; 1140204 (2020) https://doi.org/10.1117/12.2558113
Event: SPIE Defense + Commercial Sensing, 2020, Online Only

Abstract

A new branch of research, dedicated to lightfield, has recently seen an important growth in the microscopist community and it is called integral or lightfield microscopy. One recent implementation of a lightfield microscope is the Fourier integral Microscope (FiMic). In this setup a microlens array (MLA) is placed at the Fourier plane of the objective lens, therefore, the sensor behind each microlens is capturing the spatial information of a different perspective of the sample. The spatio-angular information captured can used to reconstruct the 3D volume. A very wide field of research among microscopists is for objects that have an extremely low contrast or that are completely transparent. In order to obtain a 3D reconstruction of a transparent sample our work has been focused on the combination of the FiMic with a dark-field illumination. In this way a 3D reconstruction of phase objects is achieved.

Conference Presentation

Citation Download Citation

Gabriele Scrofani, Jorge Sola-Pikabea, Emilio Sánchez-Ortiga, Manuel Martínez-Corral, and Genaro Saavedra "Light-field Fourier Microscope (FiMic) for transparent objects (Conference Presentation)", Proc. SPIE 11402, Three-Dimensional Imaging, Visualization, and Display 2020, 1140204 (22 April 2020); https://doi.org/10.1117/12.2558113

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