Direct Determination Of Conformational Disorder In Biological Membranes By FT-IR Spectroscopy

Richard Mendelsohn; Mark Davies; Herbert F. Schuster; Joseph W. Brauner; Richard A. Dluhy

doi:10.1117/12.969621

1 December 1989 Direct Determination Of Conformational Disorder In Biological Membranes By FT-IR Spectroscopy

Richard Mendelsohn, Mark Davies, Herbert F. Schuster, Joseph W. Brauner, Richard A. Dluhy

Proceedings Volume 1145, 7th Intl Conf on Fourier Transform Spectroscopy; (1989) https://doi.org/10.1117/12.969621
Event: Seventh International Conference on Fourier and Computerized Infrared Spectroscopy, 1989, Fairfax, VA, United States

Abstract

Although the determination of conformational disorder in biological membranes is an important goal of current membrane biophysics, a quantitative formulation has remained elusive. The current study describes a successful determination of trans-gauche isomerization at specific depths in phospholipid bilayers. The approach was suggested by Snyder and Poore (1) and utilizes the CD2 rocking modes of specifically deuterated hydrocarbons as conformational probes. This CD2 probe frequency is dependent on the conformation of the C-C-C skeleton of which the central carbon atom contains the CD2 moiety. The various conformations give rise to discrete (but weak) bands in the IR, as summarized below:

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Richard Mendelsohn, Mark Davies, Herbert F. Schuster, Joseph W. Brauner, and Richard A. Dluhy "Direct Determination Of Conformational Disorder In Biological Membranes By FT-IR Spectroscopy", Proc. SPIE 1145, 7th Intl Conf on Fourier Transform Spectroscopy, (1 December 1989); https://doi.org/10.1117/12.969621

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