Spatial frequency spontaneous and nonlinear Raman microscopy

Randy A. Bartels; Sandro Heuke; Siddharth Sivankutty; Camille Scotté; Patrick Stockton; Anne Sentenac; Hervé Rigneault

doi:10.1117/12.2571276

25 August 2020 Spatial frequency spontaneous and nonlinear Raman microscopy

Randy A. Bartels, Sandro Heuke, Siddharth Sivankutty, Camille Scotté, Patrick Stockton, Anne Sentenac, Hervé Rigneault

Proceedings Volume 11497, Ultrafast Nonlinear Imaging and Spectroscopy VIII; 1149712 (2020) https://doi.org/10.1117/12.2571276
Event: SPIE Optical Engineering + Applications, 2020, Online Only

Abstract

Raman microscopy has been widely developed for label free nonlinear optical microscopy of biological systems. Imaging speed in these systems is hampered by low Raman scattering cross sections and the requirement of scanning a focused laser beam through the sample in conventional Raman microscopy. The serial acquisition that is necessary in point scanning microscopy slows image acquisition and limits the dwell time are each image pixel. Here, we discuss two new imaging methods that are based on spatial frequency modulation imagining (SPIFI) [1-2], where a structured line focus is used to image is used to image specimens by collecting light on a single pixel detector. We discuss the use of SPIFI to improve the imaging speed of Spontaneous Raman scattering and coherent anti-Stokes Raman scattering microscopy. A detailed noise analysis highlighting the advantages and disadvantages of SPIFI as compared to conventional point scan imaging is presented.

Conference Presentation

Citation Download Citation

Randy A. Bartels, Sandro Heuke, Siddharth Sivankutty, Camille Scotté, Patrick Stockton, Anne Sentenac, and Hervé Rigneault "Spatial frequency spontaneous and nonlinear Raman microscopy", Proc. SPIE 11497, Ultrafast Nonlinear Imaging and Spectroscopy VIII, 1149712 (25 August 2020); https://doi.org/10.1117/12.2571276

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