The Moderate Resolution Imaging Spectroradiometer (MODIS) instrument on-board the NASA’s Earth Observing System Terra satellite has continued successful Earth-sensing operations for over 20 years. To aid in its mission in providing calibrated science data to the worldwide user community, the MODIS instrument is equipped with several on-board calibrators designed to measure changes in the instrument response over time. One such calibrator is the Spectro- Radiometric Calibration Assembly (SRCA), which can provide a source signal for radiometric, spectral, or spatial characterization. When commanded into its spatial calibration mode, the SRCA is able to produce light across the MODIS band spectral range (0.412μm to 14.2μm) at a variety of signal levels thanks to several internal halogen lamps, an IR glow bar, and a neutral density filter. This signal, used in combination with commanded sub-sample measurements of the MODIS detectors, provides a basis for determining changes in the spatial performance of the MODIS spectral bands. This work summarizes the spatial calibration process using the SRCA and presents 20 years of Terra MODIS spatial performance characterized through co-registration between MODIS bands, detectors, and focal plane assemblies. Results from pre-launch testing using the Integration and Alignment Collimator and the SRCA are incorporated in the history of the Terra MODIS mission-long spatial performance. We also note modifications to the spatial characterization methodology brought on by changes to the SRCA’s operational configuration and changes to the MODIS spectral band performance, particularly after the recovery from the safe-mode event in February 2016. Results are compared against the MODIS design specifications.