Optimal functional imaging of dendritic activity via oblique single-photon excitation of voltage indicators

Michael Castanares; Hans A. Bachor; Vincent Daria

doi:10.1117/12.2573217

15 June 2020 Optimal functional imaging of dendritic activity via oblique single-photon excitation of voltage indicators

Michael Castanares, Hans A. Bachor, Vincent Daria

Author Affiliations +

Proceedings Volume 11521, Biomedical Imaging and Sensing Conference 2020; 1152108 (2020) https://doi.org/10.1117/12.2573217
Event: SPIE Technologies and Applications of Structured Light, 2020, Yokohama, Japan

Abstract

We use patterned light to initiate scattered photons that are obliquely directed to excite voltage indicators, which report changes in the membrane potential. Our proposed scheme reduces the excitation of voltage indicators elsewhere in the cell that constitutes the background signal and therefore enables us to amplify the detected fluorescence carrying the voltage information.

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Michael Castanares, Hans A. Bachor, and Vincent Daria "Optimal functional imaging of dendritic activity via oblique single-photon excitation of voltage indicators", Proc. SPIE 11521, Biomedical Imaging and Sensing Conference 2020, 1152108 (15 June 2020); https://doi.org/10.1117/12.2573217

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