Super-resolution fluorescence assisted diffraction computational tomography reveals the three-dimensional landscape of cellular organelle interactome

Dashan Dong; Xiaoshuai Huang; Liuju Li; Kebin Shi; Liangyi Chen

doi:10.1117/12.2573177

10 October 2020 Super-resolution fluorescence assisted diffraction computational tomography reveals the three-dimensional landscape of cellular organelle interactome

Dashan Dong, Xiaoshuai Huang, Liuju Li, Kebin Shi, Liangyi Chen

Author Affiliations +

Proceedings Volume 11549, Advanced Optical Imaging Technologies III; 115490G (2020) https://doi.org/10.1117/12.2573177
Event: SPIE/COS Photonics Asia, 2020, Online Only

Abstract

The emergence of super-resolution (SR) fluorescence microscopy has rejuvenated the search for new cellular sub-structures. However, SR fluorescence microscopy achieves high contrast at the cost of the lack of a holistic view of their interacting partners and surrounding environment. Thus we develop SR fluorescence-assisted diffraction computational tomography (SR-FACT), which combines label-free three-dimensional optical diffraction tomography (ODT) with two-dimensional fluorescence Hessian structured illumination microscopy. The ODT module is capable of resolving mitochondria, lipid droplets, the nuclear membrane, chromosomes, the tubular endoplasmic reticulum and lysosomes. Using dual-mode correlated live cell imaging for prolonged period of time, we observe the dynamics of a novel subcellular structure named dark-vacuole bodies. These works demonstrate the unique capabilities of SR-FACT, which suggest its wide applicability in cell biology in general.

Conference Presentation

Citation Download Citation

Dashan Dong, Xiaoshuai Huang, Liuju Li, Kebin Shi, and Liangyi Chen "Super-resolution fluorescence assisted diffraction computational tomography reveals the three-dimensional landscape of cellular organelle interactome", Proc. SPIE 11549, Advanced Optical Imaging Technologies III, 115490G (10 October 2020); https://doi.org/10.1117/12.2573177

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