Pushing the resolution of infrared imaging by mid infrared photothermal microscopy

Pengcheng Fu; Tianrun Chen; Hanlin Zhu; Chenran Xu; Ming Chen; Delong Zhang

doi:10.1117/12.2575306

22 October 2020 Pushing the resolution of infrared imaging by mid infrared photothermal microscopy

Pengcheng Fu, Tianrun Chen, Hanlin Zhu, Chenran Xu, Ming Chen, Delong Zhang

Proceedings Volume 11549, Advanced Optical Imaging Technologies III; 115490P (2020) https://doi.org/10.1117/12.2575306
Event: SPIE/COS Photonics Asia, 2020, Online Only

Abstract

Infrared (IR) spectroscopy depicts molecular structure and dynamics based on vibrational absorption of chemical bonds. Spatially resolved IR spectroscopy, i.e. IR imaging, further enabled label-free in situ chemical imaging for dynamics in complex systems. However, IR imaging suffers from low spatial resolution at a few micrometers due to diffraction limit, thus having difficulty in applications such as sub-cellular imaging. Recently, by visible light probing of the photothermal effect of vibrational absorption, mid-infrared photothermal imaging (MIP) overcomes the limitations of conventional IR microscopy and has achieved sub-micron resolution. In this work, we built an optimized MIP system to boost the spatial resolution and sensitivity, and demonstrated MIP imaging of nanometer-sized polymeric microspheres and living cells with a high spatial resolution of 200 nm.

Conference Presentation

Citation Download Citation

Pengcheng Fu, Tianrun Chen, Hanlin Zhu, Chenran Xu, Ming Chen, and Delong Zhang "Pushing the resolution of infrared imaging by mid infrared photothermal microscopy", Proc. SPIE 11549, Advanced Optical Imaging Technologies III, 115490P (22 October 2020); https://doi.org/10.1117/12.2575306

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