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Since it was first presented in 2002, the Optical Projection Tomography(OPT) imaging system has emerged as a powerful tool for the study of a biomedical specimen on the mm to cm scale. In this paper, we present a rough and precise algorithm to further improve OPT image acquisition and tomographic reconstruction. The rough and precise algorithm combines the merits of the binarization process and the maximum correlation coefficient, and can accurately correct the displacement of the rotation axis. The tomographic images corrected by the rough and precise algorithm have higher image quality in the simulation experiments and specimen experiments. The reconstructed 3D images based on tomographic images can restore the original specimens. Thereby, the rough and precise algorithm contributes to increasing acquisition speed and quality of OPT data. More work should be performed to better understand and amend the rough and precise algorithm by abundant specimen experiments.
Ling Ma,Jihui Wang,Qingwen Tan, andGang Li
"Research on the rough and precise correction method to the rotation axis position for optical projection image systems", Proc. SPIE 11549, Advanced Optical Imaging Technologies III, 115491G (10 October 2020); https://doi.org/10.1117/12.2574784
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Ling Ma, Jihui Wang, Qingwen Tan, Gang Li, "Research on the rough and precise correction method to the rotation axis position for optical projection image systems," Proc. SPIE 11549, Advanced Optical Imaging Technologies III, 115491G (10 October 2020); https://doi.org/10.1117/12.2574784