The suppression of the side lobes of the fluorescence light-sheet microscopy with scanned Bessel beam by using the leaky filter

Zijun Ding; Luohong Hu; Liusong Yuan; Deyi Yuan; Xianhong Li; Yulong Zhang; Suhui Deng

doi:10.1117/12.2576040

10 October 2020 The suppression of the side lobes of the fluorescence light-sheet microscopy with scanned Bessel beam by using the leaky filter

Zijun Ding, Luohong Hu, Liusong Yuan, Deyi Yuan, Xianhong Li, Yulong Zhang, Suhui Deng

Author Affiliations +

Proceedings Volume 11549, Advanced Optical Imaging Technologies III; 115491S (2020) https://doi.org/10.1117/12.2576040
Event: SPIE/COS Photonics Asia, 2020, Online Only

Conference Poster

Abstract

Light-sheet Fluorescence microscopy (LSFM) with scanned Bessel beam draws a strong attention because it provides certain advantages. Especially, a thin light-sheet over a large field can be produced by Bessel beams. Also, as a non-diffracting beam, the Bessel beam enables deeper penetration into thick cells or tissues. Usually, the Bessel beam with high side-lobes is created by inserting an annular pupil filter in a Gaussian beam. The fluorescence background from the side lobes of the Bessel beam blurs the image and broadens the thickness of the light-sheet, which results in a lower image contrast and sacrificing the optical sectioning capability. To address this issue, in this paper, a leaky filter is designed to be inserted in the Gaussian beam to create a similar Bessel beam with lower side lobes. By optimizing the parameters of the leaky filter, the suppression of the side lobes is demonstrated.

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Citation Download Citation

Zijun Ding, Luohong Hu, Liusong Yuan, Deyi Yuan, Xianhong Li, Yulong Zhang, and Suhui Deng "The suppression of the side lobes of the fluorescence light-sheet microscopy with scanned Bessel beam by using the leaky filter", Proc. SPIE 11549, Advanced Optical Imaging Technologies III, 115491S (10 October 2020); https://doi.org/10.1117/12.2576040

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