Improvement and denoising for dual-wavelength digital holographic microscopy

Hongpeng Qin; Rikang Qin; Zhuqing Jiang

doi:10.1117/12.2573663

10 October 2020 Improvement and denoising for dual-wavelength digital holographic microscopy

Hongpeng Qin, Rikang Qin, Zhuqing Jiang

Proceedings Volume 11551, Holography, Diffractive Optics, and Applications X; 115511K (2020) https://doi.org/10.1117/12.2573663
Event: SPIE/COS Photonics Asia, 2020, Online Only

Conference Poster

Abstract

An optimization algorithm is developed to extend the unambiguous range of DWDH, which is suitable for imaging living onion epidermal cells, and meanwhile to reduce the noise. In the experiments, the holograms with two wavelengths can be acquired in a single shot recording by using off-axis dual-wavelength digital holography, resulting to obtain their two sets of spatial spectrum via Fourier transform for these two holograms. The denoised unwrapping phase image of sample is straightforward reconstructed with the optimization algorithm, instead of increasing the noise due to longer synthetic wavelength. As a result, the unwrapped phase images of onion epidermal cells are achieved.

Citation Download Citation

Hongpeng Qin, Rikang Qin, and Zhuqing Jiang "Improvement and denoising for dual-wavelength digital holographic microscopy", Proc. SPIE 11551, Holography, Diffractive Optics, and Applications X, 115511K (10 October 2020); https://doi.org/10.1117/12.2573663

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