Parallel acquisition of 2D multifocal Raman spectroscopy using compressive sensing

Pengfei Zhang; Guiwen Wang; Xiujuan Zhang; Yong-Qing Li

doi:10.1117/12.2570487

10 October 2020 Parallel acquisition of 2D multifocal Raman spectroscopy using compressive sensing

Pengfei Zhang, Guiwen Wang, Xiujuan Zhang, Yong-Qing Li

Proceedings Volume 11553, Optics in Health Care and Biomedical Optics X; 115531C (2020) https://doi.org/10.1117/12.2570487
Event: SPIE/COS Photonics Asia, 2020, Online Only

Abstract

Parallel confocal spectroscopy can significantly expand the analytical capacity of single biological cells and Raman hyperspectral imaging. Here, we report the development of a compressive sensing technique for single-acquisition multifocal Raman spectroscopy, which is capable of improving the speed of conventional confocal Raman spectroscopy by 2-3 orders of magnitude. The technique generates a 2-D multifocus excitation pattern and simultaneously record the Raman spectra from the multi-foci by projecting their scatterings along both the vertical and horizontal direction of the CCD camera, and both a pseudo-inverse and a hierarchical sparsity algorithms are developed to retrieve the individual spectra. The performance was validated by Raman spectroscopy of multiple trapped cells as well as by large-scale Raman imaging.

Conference Presentation

Citation Download Citation

Pengfei Zhang, Guiwen Wang, Xiujuan Zhang, and Yong-Qing Li "Parallel acquisition of 2D multifocal Raman spectroscopy using compressive sensing", Proc. SPIE 11553, Optics in Health Care and Biomedical Optics X, 115531C (10 October 2020); https://doi.org/10.1117/12.2570487

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