High-speed wide-field optical-sectioning fluorescence microscopy based on one-shot structured illumination

Zhong Zheng; Ruheng Shi; Lingjie Kong

doi:10.1117/12.2575172

10 October 2020 High-speed wide-field optical-sectioning fluorescence microscopy based on one-shot structured illumination

Zhong Zheng, Ruheng Shi, Lingjie Kong

Author Affiliations +

Proceedings Volume 11553, Optics in Health Care and Biomedical Optics X; 115532O (2020) https://doi.org/10.1117/12.2575172
Event: SPIE/COS Photonics Asia, 2020, Online Only

Conference Poster

Abstract

Wide-field fluorescence microscopy (WFFM) is widely adopted in biomedical studies. However, axial resolution in most WFFM is poor due to the absence of optical-sectioning capability. To achieve wide-field optical-sectioning, several methods have been proposed, most of which need at least two images to reconstruct one optical sectioning image. So, the frame rate of current wide-field optical sectioning microscopy is no more than half of that of conventional WFFM, which may not meet the speed requirement of fast biodynamic studies. We introduce a novel high-speed, wide-field optical sectioning method based on local contrast weighting function and two-dimensional Hilbert-Huang transform, in which only one structured image is required to reconstruct an optical sectioning image. In this way, the loss of temporal resolution in conventional wide-field optical sectioning microscopy is compensated. We validated this method with the imaging of mouse brain slices.

Conference Presentation

Citation Download Citation

Zhong Zheng, Ruheng Shi, and Lingjie Kong "High-speed wide-field optical-sectioning fluorescence microscopy based on one-shot structured illumination", Proc. SPIE 11553, Optics in Health Care and Biomedical Optics X, 115532O (10 October 2020); https://doi.org/10.1117/12.2575172

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