HSP47 expression in fibroblasts by NIR laser irradiation in vitro

Sota Kawakami; Emiyu Ogawa; Hiroshi Kumagai

doi:10.1117/12.2577466

5 March 2021 HSP47 expression in fibroblasts by NIR laser irradiation in vitro

Sota Kawakami, Emiyu Ogawa, Hiroshi Kumagai

Proceedings Volume 11618, Photonics in Dermatology and Plastic Surgery 2021; 1161806 (2021) https://doi.org/10.1117/12.2577466
Event: SPIE BiOS, 2021, Online Only

Abstract

We investigated the photothermal effect of near-infrared pulsed laser irradiation on collagen synthesis in fibroblasts in vitro to reduce complications in skin rejuvenation therapy by selective collagen heating. Tissue thermal damage with pain by laser heating of water is a problem in conventional laser skin rejuvenation therapy. To reduce these complications, we suggest heating collagen fibril selectively by applying selective photothermolysis. Laser parameters such as wavelength, pulse duration, pulse interval, and irradiance are important for selective heating. Fibroblasts will be heated gently by conducted heat from collagen fibril in our process, which enhances the expression of heat shock protein 47 (HSP47) in fibroblasts. This procedure would reduce pain and thermal damage on fibroblasts compared with the conventional procedure. In this study, we evaluated the amount of HSP47 content per living cell to investigate the photothermal effect on fibroblasts. The temperature distribution of the dermis during laser irradiation was simulated by the finite element method (FEM). 1480 and 1550 nm pulsed laser was irradiated to cultured fibroblasts. The laser emitted with a pulse duration of 100 μs, repetition of 2 kHz, fluence of 0 - 60 J/cm² , and irradiance of 0.5 W/cm² . To evaluate cell functional changes in time, ELISA and cell viability assay were performed 24 and 48 hours after laser irradiation.

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Citation Download Citation

Sota Kawakami, Emiyu Ogawa, and Hiroshi Kumagai "HSP47 expression in fibroblasts by NIR laser irradiation in vitro", Proc. SPIE 11618, Photonics in Dermatology and Plastic Surgery 2021, 1161806 (5 March 2021); https://doi.org/10.1117/12.2577466

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