Feature enhancement volumetric visualization of human brain sample with serial sectioning spectral domain optical coherence tomography

Jiarui Yang; Shuaibin Chang; Ichun Anderson Chen; Divya Varadarajan; Hui Wang; Caroline Magnain; Bruce Fischl; Irene Costantini; Francesco Pavone; Ann C. McKee; Shih-Chi Chen; David A. Boas

doi:10.1117/12.2577771

5 March 2021 Feature enhancement volumetric visualization of human brain sample with serial sectioning spectral domain optical coherence tomography

Jiarui Yang, Shuaibin Chang, Ichun Anderson Chen, Divya Varadarajan, Hui Wang, Caroline Magnain, Bruce Fischl, Irene Costantini, Francesco Pavone, Ann C. McKee, Shih-Chi Chen, David A. Boas

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Proceedings Volume 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics; 116290Y (2021) https://doi.org/10.1117/12.2577771
Event: SPIE BiOS, 2021, Online Only

Abstract

The signal of optical coherence tomography (OCT) decays exponentially in depth due to tissue scattering, resulting in indistinct tissue features in three-dimension. Moreover, due to limited light penetration depth, extensive volumetric investigation is usually constrained for large-scale biological samples. By integrating serial sectioning technology with block-face imaging, we establish a volumetric OCT acquisition and reconstruction pipeline that incorporates depth-resolved attenuation coefficient estimation, volumetric stitching and filtering, and feature enhancement visualization. We demonstrate this pipeline on ex vivo human brain volumes of several cubic centimeters with 5 um isotropic resolution.

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Jiarui Yang, Shuaibin Chang, Ichun Anderson Chen, Divya Varadarajan, Hui Wang, Caroline Magnain, Bruce Fischl, Irene Costantini, Francesco Pavone, Ann C. McKee, Shih-Chi Chen, and David A. Boas "Feature enhancement volumetric visualization of human brain sample with serial sectioning spectral domain optical coherence tomography", Proc. SPIE 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 116290Y (5 March 2021); https://doi.org/10.1117/12.2577771

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KEYWORDS

Optical coherence tomography

Brain

Visualization

Signal attenuation

Tissue optics

Scattering

Signal to noise ratio

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