Neuroprotection by optical delivery of therapeutic gene into retina

Subrata Batabyal; Sanghoon Kim; Michael Carlson; Houssam Al-Saad; Jazlyn Gallego; Adnan Dibas; Samarendra K. Mohanty

doi:10.1117/12.2586104

5 March 2021 Neuroprotection by optical delivery of therapeutic gene into retina

Subrata Batabyal, Sanghoon Kim, Michael Carlson, Houssam Al-Saad, Jazlyn Gallego, Adnan Dibas, Samarendra K. Mohanty

Proceedings Volume 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics; 1162916 (2021) https://doi.org/10.1117/12.2586104
Event: SPIE BiOS, 2021, Online Only

Abstract

We hypothesized that PEDF gene transduction in retina can provide single-dose treatment to prevent ganglion cell damage. Here, we present OCT guided ultrafast laser based non-viral targeted delivery PEDF-encoding genes to retina for neuroprotection. The ultrafast laser gene delivery showed layer-specific reliable expression of PEDF gene in retina without any detectable damage. Monitoring of IOP and electroretinogram after ultrafast laser transfection showed no adverse changes. The ultrafast laser transfection of large PEDF genes in retina exhibited significant therapeutic benefit in an injury model. Absence of any immune response in retina subsequent to ultrafast-laser transfection provides unique opportunity for repeated dosing.

Conference Presentation

Citation Download Citation

Subrata Batabyal, Sanghoon Kim, Michael Carlson, Houssam Al-Saad, Jazlyn Gallego, Adnan Dibas, and Samarendra K. Mohanty "Neuroprotection by optical delivery of therapeutic gene into retina", Proc. SPIE 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 1162916 (5 March 2021); https://doi.org/10.1117/12.2586104

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available