Photothermal effects and functional neuroimaging using large field two-photon microscopy in mice

Hunter B. Banks; Jon R. Bumstead; Lindsey M. Brier; Annie Bice; Seana Gaines; Joseph P. Culver

doi:10.1117/12.2578578

5 March 2021 Photothermal effects and functional neuroimaging using large field two-photon microscopy in mice

Hunter B. Banks, Jon R. Bumstead, Lindsey M. Brier, Annie Bice, Seana Gaines, Joseph P. Culver

Proceedings Volume 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics; 116291X (2021) https://doi.org/10.1117/12.2578578
Event: SPIE BiOS, 2021, Online Only

Abstract

By paying careful attention to optical and mechanical design, a two-photon microscope (TPM) can image a large field of view (FOV) with high spatial and temporal resolution. Successfully applying such a TPM for functional neuroimaging requires careful consideration of the signal properties, physiological limitations, and image processing techniques. We present our thermal imaging results as well as functional imaging results in awake mice using a fast, large FOV TPM, and discuss how the system expands the spatial bandwidth available for studying functional connectivity.

Conference Presentation

Citation Download Citation

Hunter B. Banks, Jon R. Bumstead, Lindsey M. Brier, Annie Bice, Seana Gaines, and Joseph P. Culver "Photothermal effects and functional neuroimaging using large field two-photon microscopy in mice", Proc. SPIE 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 116291X (5 March 2021); https://doi.org/10.1117/12.2578578

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