Single-shot 4-phase shifting optical coherence microscopy

Mantas Žurauskas; Rishyashring R. Iyer; Stephen A. Boppart

doi:10.1117/12.2583583

5 March 2021 Single-shot 4-phase shifting optical coherence microscopy

Mantas Žurauskas, Rishyashring R. Iyer, Stephen A. Boppart

Proceedings Volume 11630, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXV; 1163019 (2021) https://doi.org/10.1117/12.2583583
Event: SPIE BiOS, 2021, Online Only

Abstract

One limitation of phase shifting optical coherence microscopy (OCM) is that any changes in imaging conditions, caused by either system-level instability or movement of dynamic samples, will inevitably lead to reduction in image quality. Here we present a novel implementation of OCM which permits accurate measurements of phase and intensity, by simultaneously capturing four phase-shifted images on a single camera sensor. Uniquely, our approach enables probing the sample with unpolarized light, producing four phase-shifted interferograms, separated by π2 in two orthogonally polarized detection channel pairs. The proposed set-up is compatible with spatially incoherent illumination sources, enabling high resolution imaging due to its inherently reduced susceptibility to speckle noise. By simultaneously capturing the four phase-shifted images on a single CCD camera chip we demonstrate the capacity to acquire stable phase recordings with a phase sensitivity of 0.1 nm rms error at 500 Hz frame rates, a field-of-view of 250x250 μm, and 1μm lateral resolution. Instantaneous, coherence-gated, phase-sensitive imaging at high frame rates enables observations of the dynamics in cells and tissues, providing instant feedback on metabolic activity and tissue states. We present the novel design and methodology that enables ultrasensitive label-free coherence-gated measurements of intracellular dynamics in cells and cellular networks.

Conference Presentation

Citation Download Citation

Mantas Žurauskas, Rishyashring R. Iyer, and Stephen A. Boppart "Single-shot 4-phase shifting optical coherence microscopy", Proc. SPIE 11630, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXV, 1163019 (5 March 2021); https://doi.org/10.1117/12.2583583

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available