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5 March 2021 Toward a multi-modal Raman and fluorescence spectroscopic platform at the point-of-care
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Abstract
Point-of-care (PoC) devices are gaining more attention due to their rapid readout times, low cost and reduced system complexity. The use of these devices to aid in health outcomes, particularly of populations that lack access to centralized healthcare, is essential to improving quality of life. Broad fluorescence background presents a major issue in Raman detection with suppression techniques being employed to prevent signal crosstalk. The presented spectroscopic platform splits the Raman and fluorescence signal onto separate detectors in order in minimize crosstalk and to extract useful information from each optical signal. Separate illumination sources were utilized to selectively excite either Raman or fluorescence emission. The developed spectroscopic platform was designed using off-the-shelf components with small form factor and ultimately the capacity for low cost being the primary selection criteria. The platform enables multimodal detection of Raman signal over a spectral range of 900 – 2000 cm-1 with a resolution of 2 nm, coupled with monitoring the average fluorescence emission intensity. The ratio of the two signals is compared in order to quantify the concentration of target molecules present. The optical system was assembled on a portable optical breadboard and calibrated using an Argon emission lamp. The multimodal functionality was validated using Raman reporter (4-MBA) tagged gold nanoparticles in solution with unbound fluorophores (fluorescein). Results showed an increase in both the Raman and fluorescence signals as the concentration of each was increased from 5-55 μM.
Conference Presentation
© (2021) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Cyril Soliman, Sureyya Paterson, Michael McShane, Gerard Coté, and Kristen Maitland "Toward a multi-modal Raman and fluorescence spectroscopic platform at the point-of-care", Proc. SPIE 11632, Optics and Biophotonics in Low-Resource Settings VII, 116320M (5 March 2021); https://doi.org/10.1117/12.2578945
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