Sectioning-free virtual H&E histology with fiber-based two-photon microscopy

Matthias Strauch; Jan Philip Kolb; Wolfgang Draxinger; Ann-Kathrin Popp; Melanie Wacker; Nadine Merg; Jennifer Hundt; Sebastian Karpf; Robert Huber

doi:10.1117/12.2578334

5 March 2021 Sectioning-free virtual H&E histology with fiber-based two-photon microscopy

Matthias Strauch, Jan Philip Kolb, Wolfgang Draxinger, Ann-Kathrin Popp, Melanie Wacker, Nadine Merg, Jennifer Hundt, Sebastian Karpf, Robert Huber

Author Affiliations +

Proceedings Volume 11648, Multiphoton Microscopy in the Biomedical Sciences XXI; 116480V (2021) https://doi.org/10.1117/12.2578334
Event: SPIE BiOS, 2021, Online Only

Abstract

The routine pathology workflow relies on cutting tissue into single-cell layer thick slices using paraffin or frozen sectioning. We propose a fast method to obtain sections of equivalent quality optically using the strong sectioning capabilities of two-photon microscopy (TPM). Hematoxylin and eosin (HE) equivalent staining of the tissue is achieved using acridine orange and sulforhodamine 101. We improved our previously presented pulsed fiber laser to deliver adjustable pulse durations of ~30ps at repetition rates of up to 16MHz and kW peak power. We can now image up to one square centimeter of tissue with sub-micrometer resolution within 15 minutes.

Conference Presentation

Citation Download Citation

Matthias Strauch, Jan Philip Kolb, Wolfgang Draxinger, Ann-Kathrin Popp, Melanie Wacker, Nadine Merg, Jennifer Hundt, Sebastian Karpf, and Robert Huber "Sectioning-free virtual H&E histology with fiber-based two-photon microscopy", Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, 116480V (5 March 2021); https://doi.org/10.1117/12.2578334

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