The transition dipole moment representation and spectral phasors

Jessica Kline; Marcos Dantus

doi:10.1117/12.2582780

5 March 2021 The transition dipole moment representation and spectral phasors

Jessica Kline, Marcos Dantus

Proceedings Volume 11648, Multiphoton Microscopy in the Biomedical Sciences XXI; 1164813 (2021) https://doi.org/10.1117/12.2582780
Event: SPIE BiOS, 2021, Online Only

Abstract

Spectral phasors are currently based on fluorescence spectra in the wavelength domain. However, this is a problem when using spectral phasors to quantify emitters since wavelength represented spectra are not proportional to emitter population. Here we show the effect of unmixing using spectra in their transition dipole moment representation for a theoretical two component system and on a real seven component system.

Conference Presentation

Citation Download Citation

Jessica Kline and Marcos Dantus "The transition dipole moment representation and spectral phasors", Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, 1164813 (5 March 2021); https://doi.org/10.1117/12.2582780

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