Limitations of Fourier ring correlation as an image resolution metric

Erik K. Johnson; Stephen Becker; Carol Cogswell; Jian Xing; Simeng Chen

doi:10.1117/12.2585852

5 March 2021 Limitations of Fourier ring correlation as an image resolution metric

Erik K. Johnson, Stephen Becker, Carol Cogswell, Jian Xing, Simeng Chen

Proceedings Volume 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII; 1164909 (2021) https://doi.org/10.1117/12.2585852
Event: SPIE BiOS, 2021, Online Only

Abstract

Fourier ring correlation was recently proposed in fluorescence microscopy as an objective measure of image resolution. In this study we show that FRC has serious limitations. For several common image processing techniques, we show that FRC can be made to be systematically biased to classify objectively lower resolution images as better resolved than objectively higher resolution images. Thus, rather than providing an objective, realistic measure of spatial resolution, care must be taken in interpreting FRC-based results. In revealing some of FRC’s limitations, this study also highlights the need for a more objective, principled approach to estimating resolution.

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Erik K. Johnson, Stephen Becker, Carol Cogswell, Jian Xing, and Simeng Chen "Limitations of Fourier ring correlation as an image resolution metric", Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 1164909 (5 March 2021); https://doi.org/10.1117/12.2585852

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KEYWORDS

Image resolution

Luminescence

Microscopy

Spatial resolution

Image analysis

Image denoising

Image processing

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