Nanoscale nuclear architecture mapping captures chromatin reorganization in epithelial cells undergoing malignant transformation

Shikhar Uttam; Jianquan Xu; Yang Liu

doi:10.1117/12.2583147

5 March 2021 Nanoscale nuclear architecture mapping captures chromatin reorganization in epithelial cells undergoing malignant transformation

Shikhar Uttam, Jianquan Xu, Yang Liu

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Proceedings Volume 11653, Quantitative Phase Imaging VII; 1165318 (2021) https://doi.org/10.1117/12.2583147
Event: SPIE BiOS, 2021, Online Only

Abstract

Malignant transformation of normal epithelial cells involves extensive epigenetic reprogramming that alters the structure of chromatin within the cell nuclei. We have shown that Fourier phase associated with nanoscale nuclear architecture mapping (nanoNAM) of epithelial cells based on Fourier-domain optical coherence tomography (FD-OCT) can capture the resulting changes in nuclear architecture via nanoscale-sensitive joint characterization of coherence-gated mean alteration in nuclear refractive index and its associated heterogeneity. Here, using computational modeling and stochastic optical reconstruction microscopy (STORM) based super-resolution imaging, we demonstrate the underlying structural changes of chromatin remodeling during malignant transformation in epithelial cells measured by depth-resolved nanoNAM.

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Shikhar Uttam, Jianquan Xu, and Yang Liu "Nanoscale nuclear architecture mapping captures chromatin reorganization in epithelial cells undergoing malignant transformation", Proc. SPIE 11653, Quantitative Phase Imaging VII, 1165318 (5 March 2021); https://doi.org/10.1117/12.2583147

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KEYWORDS

Tumor growth modeling

Computational modeling

Computer simulations

Image analysis

Mouse models

Optical coherence tomography

Refractive index

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