High resolution phase imaging with transport of intensity equation and sinusoidal illumination

Sibi Chakravarthy Shanmugavel; Yunhui Zhu

doi:10.1117/12.2579647

5 March 2021 High resolution phase imaging with transport of intensity equation and sinusoidal illumination

Sibi Chakravarthy Shanmugavel, Yunhui Zhu

Proceedings Volume 11653, Quantitative Phase Imaging VII; 116531K (2021) https://doi.org/10.1117/12.2579647
Event: SPIE BiOS, 2021, Online Only

Conference Poster

Abstract

Transport of Intensity equation(TIE) is a non-interferometric method used for quantitative phase imaging. By reformulating the TIE using Contrast Transfer Function, it can be determined that the spatial resolution of the phase retrieved using TIE is limited by the product of imaging system transfer function and a sinc function. In this work, we apply the principles of structured illumination fluorescent microscopy to develop a TIE based super resolved phase imaging technique. The sinusoidal intensity pattern down modulates high frequency spectrum of the phase into the system pass band thereby providing a convenient approach to synthetically enlarge the numerical aperture of the system. Resolution enhancement by two folds is demonstrated using simulations.

Conference Presentation

Citation Download Citation

Sibi Chakravarthy Shanmugavel and Yunhui Zhu "High resolution phase imaging with transport of intensity equation and sinusoidal illumination", Proc. SPIE 11653, Quantitative Phase Imaging VII, 116531K (5 March 2021); https://doi.org/10.1117/12.2579647

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available