Quantitative phase imaging of plant cells

Xin Shu; Yonglun Zeng; Liwen Jiang; Renjie Zhou; Yanping He

doi:10.1117/12.2579117

7 March 2021 Quantitative phase imaging of plant cells

Xin Shu, Yonglun Zeng, Liwen Jiang, Renjie Zhou, Yanping He

Proceedings Volume 11653, Quantitative Phase Imaging VII; 116531T (2021) https://doi.org/10.1117/12.2579117
Event: SPIE BiOS, 2021, Online Only

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Abstract

Observation of living plant cells under conventional brightfield microscopy suffers from low imaging contrast. Therefore, fluorescence labeling or fluorescence tag is typically required, but it may not reflect the bona fide state of cellular events and prevent long-term observation due to photobleaching of the fluorescence signal. Therefore, we propose to use quantitative phase imaging (QPI) for label-free imaging of plant cell structures. Using QPI, we have observed vacuoles and nucleus in tobacco BY-2 cells, Arabidopsis cell suspension culture PSB-D, and pollen tubes.

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Xin Shu, Yonglun Zeng, Liwen Jiang, Renjie Zhou, and Yanping He "Quantitative phase imaging of plant cells", Proc. SPIE 11653, Quantitative Phase Imaging VII, 116531T (7 March 2021); https://doi.org/10.1117/12.2579117

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