Multispectral lensless imaging in the mid-infrared for label-free identification of Staphylococcus species

Joel Le Galudec; Mathieu Dupoy; Pierre Marcoux

doi:10.1117/12.2578264

5 March 2021 Multispectral lensless imaging in the mid-infrared for label-free identification of Staphylococcus species

Joel Le Galudec, Mathieu Dupoy, Pierre Marcoux

Proceedings Volume 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021; 116551E (2021) https://doi.org/10.1117/12.2578264
Event: SPIE BiOS, 2021, Online Only

Abstract

Identifying the microbial species present in a sample is critical in healthcare, industry, ecology and even national security. The traditional diagnosis process involves the growth of colonies of microorganisms on a solid-state medium in a Petri dish. This step provides great amounts of biological material, allows spatial separation of the different microorganisms and can help to visually discriminate potentially harmful species. However, it does not allow a precise identification and thereby requires further sample preparation and analysis to offer a proper diagnosis. Here, a new optical-based Petri dish analysis technology is discussed. This technique, called lensless multispectral mid-infrared imaging, relies on the acquisition of images at nine wavelengths corresponding to relevant chemical functions. It provides both morphological and discrete spectral data, which allows to discriminate even closely related species. The instrument is simply made of a laser source (four quantum cascade lasers) and a microbolometer array as the imager. A total of 1050 colonies belonging to three Staphylococcus species and two strains of Staphylococcus epidermidis have been acquired. After feature extraction and classification by Support Vector Machine, the tenfold cross-validation test yields a correct identification rate between 93 % and 96 %, with only a 5 % confusion between the two strains of S. epidermidis. Further work on the data analysis algorithms could dramatically improve these already promising results. Therefore, considering its label-free and non-destructive aspects, as well as its absence of secondary sample preparation, this technology has a great potential to offer precise Petri-dish based diagnosis.

Conference Presentation

Citation Download Citation

Joel Le Galudec, Mathieu Dupoy, and Pierre Marcoux "Multispectral lensless imaging in the mid-infrared for label-free identification of Staphylococcus species", Proc. SPIE 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021, 116551E (5 March 2021); https://doi.org/10.1117/12.2578264

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KEYWORDS

Multispectral imaging

Mid-IR

Biological research

Chemical analysis

Microorganisms

Quantum cascade lasers

Solid state physics

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