Brillouin imaging for noncontact and label-free 2D and 3D mapping of cellular mechanical properties

Maria A. Troyanova-Wood; Zachary Coker; Joel Bixler; Bennett Ibey

doi:10.1117/12.2578835

5 March 2021 Brillouin imaging for noncontact and label-free 2D and 3D mapping of cellular mechanical properties

Maria A. Troyanova-Wood, Zachary Coker, Joel Bixler, Bennett Ibey

Proceedings Volume 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021; 116551F (2021) https://doi.org/10.1117/12.2578835
Event: SPIE BiOS, 2021, Online Only

Abstract

Cell mechanical properties may undergo changes as a response to external stimuli or altered environment. Being able to map the mechanical properties over an entire cell in a contact-free and label-free manner is crucial to avoid affecting the measurements. Brillouin imaging is a non-invasive all-optical modality that can provide a distribution map of high-frequency elastic modulus and viscosity. In this report we first provide an overview of prior Brillouin cell imaging studies. We then demonstrate viscoelastic 2D and 3D cell imaging capabilities of a molecular filter-based Brillouin imaging system, with a first-time imaging application of multi-excitation (ME) Brillouin elastography.

Conference Presentation

Citation Download Citation

Maria A. Troyanova-Wood, Zachary Coker, Joel Bixler, and Bennett Ibey "Brillouin imaging for noncontact and label-free 2D and 3D mapping of cellular mechanical properties", Proc. SPIE 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021, 116551F (5 March 2021); https://doi.org/10.1117/12.2578835

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